Publications

2023

Cellular Aging Secretes: a Comparison of Bone-Marrow-Derived and Induced Mesenchymal Stem Cells and Their Secretome Over Long-Term Culture.

Marote A, Santos D, Mendes-Pinheiro B, Serre-Miranda C, Anjo SI, Vieira J, Ferreira-Antunes F, Correia JS, Borges-Pereira C, Pinho AG, Campos J, Manadas B, Teixeira MR, Correia-Neves M, Pinto L, Costa PM, Roybon L, Salgado AJ.

Stem Cell Rev Rep.

2022

Canonical Bone Morphogenetic Protein signaling regulates expression of Aquaporin-4 and its anchoring complex in mouse astrocytes

Nadia Skauli, Ekaterina Savchenko, Ole Petter Ottersen, Laurent Roybon, Mahmood Amiry-Moghaddam

Frontiers Cellular Neuroscience

Transcriptomic analysis of Parkinson’s disease and multiple system atrophy patient iPSC-derived oligodendrocytes reveal changes in maturation and immune reactive properties induced by alpha-synuclein

C. Azevedo, G. Teku, Y. Pomeshchik, J.F. Reyes, M. Chumarina, K. Russ, E. Savchenko, A. Hammarberg, N.J. Lamas, A. Collin, G.K. Gouras, O. Klementieva, M. Hallbeck, R Taipa, M. Vihinen, L. Roybon

PNAS

2021

The intracellular milieu of Parkinson’s disease patient brain cells modulates alpha‑synuclein protein aggregation

N. Gustavsson; E. Savchenko; O. Klementieva; L. Roybon

Acta Neuropathologica Communications

Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome

U.-K. Peteri; J. Pitkonen; I. de Toma; O. Nieminen; K. Utami; T. Strandin; P. Corcoran; L. Roybon; A. Vaheri; I. Ethell; P. Casarotto; M. Pouladi; M. Castren

Glia

TNFα and α-synuclein fibrils differently regulate human astrocyte immune reactivity and impair mitochondrial respiration

K. Russ; G. Teku; L. Bousset; V. Redeker; S. Piel; E. Savchenko; Y. Pomeshchik; J. Savistchenko; T.C. Stummann; C. Azevedo; A. Collin; S. Goldwurm; K. Fog; E. Elmer; M. Vihinen; R. Melki; L. Roybon

Cell Reports

Generation of human pluripotent stem cell-derived astrocyte model with forebrain identity

U.-K. Peteri, J. Pitkonen, K.H. Utami, J. Paavola, L. Roybon, M. Pouladi, M.L. Castrén

Brain Sciences

2020

Unique signatures of stress-induced senescent human astrocytes

K. Simmnacher, F. Krach, Y. Schneider, J. Alecu, L. Mautner, P. Klein, L. Roybon, I. Prots, W. Xiang, B. Winner.

Experimental Neurology

Human iPSC-derived hippocampal spheroids: an innovative tool for stratifying Alzheimer's disease patient specific cellular phenotypes and developing therapies

Y. Pomeshchik, O. Klementieva, J. Gil, I. Martinsson, M. Grønning Hansen, T. de Vries, A. Sancho-Balsells, K. Russ, E. Savchenko, A Collin, A.R. Vaz, S. Bagnoli, B. Nacmias, C. Rampon, S. Sorbi, D. Brites, G. Marko-Varga, Z. Kokaia, M. Rezeli, G.K. Gouras, L. Roybon

Stem Cell Reports

Demonstration of brain region-specific neuronal vulnerability in human iPSC-based model of familial Parkinson's disease.

Brazdis RM, Alecu J, Marsch D, Dahms A, Simmnacher K, Lörentz S, Brendler A, Schneider Y, Marxreiter F, Roybon L, Winner B, Xiang W, Prots I.

Human Molecular Genetics

NRF2 activation boosts antioxidant defenses and ameliorates inflammatory and amyloid properties in human Presenilin-1 mutated Alzheimer’s disease astrocytes

M. Oksanen, I. Hyötyläinen, K. Trontti, T. Rolova, S. Wojciechowski, M. Koskuvi, M. Viitanen, A.-L. Levonen, I. Hovatta, L. Roybon, K.M. Kanninen, Š. Lehtonen, R.H. Hämäläinen, J. Koistinaho^.

Glia

Generation of an induced pluripotent stem cell line (CSC-32) from a patient with Parkinson's disease carrying a heterozygous variation p.A53T in the SNCA gene

C. Azevedo, M. Chumarina, E. Serafimovaa, S. Goldwurm, A. Collin, L. Roybon, E. Savchenko, Y. Pomeshchik

Stem Cell Research

2019

Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene

M. Chumarina, K. Russ, C. Azevedo, A. Heuer, M. Pihl, A. Collin, E. Åsander Frostner, E. Elmer, P. Hyttel, G. Cappelletti, M. Zini, S. Goldwurm, L. Roybon

Acta Neuropathologica Communications

FGF family members differentially regulate maturation and proliferation of stem cell-derived astrocytes

E. Savchenko, G. N. Teku, A. Boza-Serrano, K. Russ, M. Berns, T. Deierborg, N. J. Lamas, H. Wichterle, J. Rothstein, C. E. Henderson, M. Vihinen, L. Roybon

Scientific Reports

PARK2 mutation causes metabolic disturbances and impaired survival of human iPSC-derived neurons

H. Bogetofte, P. Jensen, M. Ryding, S. Ida Schmidt, J. Okarmus, M. Christine Hohnholt, C. Azevedo, L. Roybon, L. Kristoffer Bak, H. Waagepetersen, B. James Ryan, R. Wade-Martins, M. Røssel Larsen, M. Meyer

Frontiers in Cellular Neuroscience

Generation of an induced pluripotent stem cell line (CSC-46) from a patient with Parkinson's disease carrying a novel p.R301C mutation in the GBA gene

N. Gustavsson, A. Marote, Y. Pomeshchik, K. Russ, C. Azevedo, M. Chumarina, S. Goldwurm, A. Collin, L. Pinto, A.J. Salgado, O. Klementieva, L. Roybon, E. Savchenko

Stem Cell Research

2018

Early postnatal behavioral, cellular, and molecular changes in models of Huntington disease are reversible by HDAC inhibition

F.A. Siebzehnrübl, K.A. Raber, Y.K. Urbach, A. Schulze-Krebs, F. Canneva, S. Moceri, J. Habermeyer, D. Achoui, B. Gupta, D.A. Steindler, M. Stephan, H. Phuc Nguyen, M. Bonin, O. Riess, A. Bauer, L. Aigner, S. Couillard-Despres, M. Arce Paucar, P. Svenningsson, A. Osmand, A. Andreew, C. Zabel, A. Weiss, R. Kuhn, S. Moussaoui, I. Blockx, A. Van der Linden, R.Y. Cheong, L. Roybon, Å. Petersén, and S. von Hörsten

PNAS

Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

A. Marote, Y. Pomeshchik, A. Collin, S. Goldwurm, N. Lamas, L. Pinto, A.J. Salgado, L Roybon

Stem Cell Research

Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson’s disease

E. Savchenko, A. Marote, K. Russ, A. Collin, S. Goldwurm, L. Roybon, Y. Pomeshchik

Stem Cell Research

Generation of an induced pluripotent stem cell line (CSC-44) from a Parkinson’s disease patient carrying a compound heterozygous mutation (c.823C>T and EX6 del) in the PARK2 gene

A. Marote, Y. Pomeshchik, S. Goldwurm, A. Collin, N. Lamas, L. Pinto, A.J. Salgado, L Roybon

Stem Cell Research

Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson’s disease

A. Marote, Y. Pomeshchik, S. Goldwurm, A. Collin, N. Lamas, L. Pinto, A.J. Salgado, L. Roybon

Stem Cell Research

Generation of a human induced pluripotent stem cell line (CSC-40) from a Parkinson’s disease patient with a PINK1 p.Q456X mutation

K. Russ, A. Marote, E. Savchenko, A. Collin, S. Goldwurm, Y. Pomeshchik, L. Roybon

Stem Cell Research

Parkin absence accelerates microtubule aging in dopaminergic neurons.

D. Cartelli, A. Amadeo, A.M. Calogero, F.V. Casagrande, C. De Gregorio, M. Gioria, N. Kuzumaki, I. Costa, J. Sassone, A. Ciammola, N. Hattori; H. Okano, S. Goldwurm, L. Roybon, G. Pezzoli, G. Cappelletti

Neurobiology of Aging

2017

Human astrocytes transfer aggregated alpha-synuclein via tunneling nanotubes

J. Rostami, S. Holmqvist, V. Lindström, J. Sigvardson, G. Westermark, M. Ingelsson, J. Bergström, L. Roybon, and A. Erlandsson

The Journal of Neuroscience

Differential alteration of hippocampal function and plasticity in females and males of the APPxPS1 mouse model of Alzheimer's disease.

K. Richetin, P. Petsophonsakul, L. Roybon, B. Guiard, C. Rampon.

Neurobiology of Aging

Amplifying mitochondrial function rescues adult neurogenesis in a mouse model of Alzheimer's disease

K. Richetin; M. Moulis; A. Millet; M. Arrazola; T. Andraini; J. Hua; N. Davezac; L. Roybon; P. Belenguer; M.-C. Miquel; C. Rampon

Neurobiology of Disease

Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome

V.S. Achuta, H. Grym, N. Putkonen, V. Louhivuori, V. Kärkkäinen, J. Koistinaho, L. Roybon, M.L. Castrén

Developmental Neurobiology

Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources

M. Djelloul, C. Azevedo, Y. Pomeshchik, A. Hammarberg and L. Roybon

Stem Cell Research

Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease

M. Chumarina, C. Azevedo, J. Bigarreau, C. Vignon, K.-S. Kim, J.-Y. Li, L. Roybon

Stem Cell Research

Memory formation orchestrates the wiring of adult-born hippocampal neurons into brain circuits

P. Petsophonsakul, K. Richetin, T. Andraini, L. Roybon, and C. Rampon

Brain Structure and Function

2016

α-Synuclein is a Novel Microtubule Dynamase

D. Cartelli, A. Aliverti, A. Barbiroli, C. Santambrogio, E.M. Ragg, F. Casagrande, F. Cantele, S. Beltramone, C. De Gregorio, J. Marangon, V. Pandini, M. Emanuele, E. Chieregatti, S. Pieraccini, S. Holmqvist, L. Bubacco, L. Roybon, G. Pezzoli, R. Grandori, I. Arnal, G. Cappelletti

Scientific Reports

Creation of a library of induced pluripotent stem cells from Parkinsonian patients

S. Holmqvist, Š. Lehtonen, M. Chumarina, K.A. Puttonen, C. Azevedo, L. Olga, M. Ruponen, M. Oksanen, M. Djelloul, A. Collin, S. Goldwurm, M. Meyer, M. Lagarkova, S. Kiselev, J. Koistinaho, L. Roybon

NPJ Parkinson's Disease

Label-free concentration of viable neurons, hESC and cancer cells by means of acoustophoresis

M.C. Zalis, J.F. Reyes, P. Augustsson, S. Holmqvist, L. Roybon, T. Laurell, T. Deierborg.

Integrative Biology

2015

Alpha-synuclein expression in the oligodendrocyte lineage: an in vitro and in vivo study using rodent and human models

Djelloul M., Holmqvist S., Boza-Serrano A., Azevedo C., Yeung M.S., Goldwurm S., Frisén J., Deierborg T., Roybon L.

Stem Cell Reports

Generation of human pluripotent stem cell reporter lines for the isolation of and reporting on astrocytes generated from ventral midbrain and ventral spinal cord neural progenitors

Holmqvist S; Brouwer M; Djelloul M; Diaz A; Devine M; Hammarberg A; Fog K; Kunath T; Roybon L.

Stem Cell Research

The Origin, Development and Molecular Diversity of Rodent Olfactory Bulb Glutamatergic Neurons Distinguished by Expression of Transcription Factor NeuroD1.

Roybon L, Mastracci TL, Li J, Stott SR, Leiter AB, Sussel L, Brundin P, Li JY.

PLoS One

Genetic manipulation of adult-born hippocampal neurons rescues memory in a mouse model of Alzheimer's disease.

Richetin K, Leclerc C, Toni N, Gallopin T, Pech S, Roybon L, Rampon C.

Brain

2014

Neurotrophic requirements of human motor neurons defined using amplified and purified stem cell-derived cultures.

Lamas NJ, Johnson-Kerner B, Roybon L, Kim YA, Garcia-Diaz A, Wichterle H, Henderson CE.

PLoS One

Direct evidence of Parkinson pathology spread from the gastrointestinal tract to the brain in rats.

Holmqvist S, Chutna O, Bousset L, Aldrin-Kirk P, Li W, Björklund T, Wang ZY, Roybon L, Melki R, Li JY.

Acta Neuropathol.

Gene profiling of human induced pluripotent stem cell-derived astrocyte progenitors following spinal cord engraftment.

Haidet-Phillips AM, Roybon L, Gross SK, Tuteja A, Donnelly CJ, Richard JP, Ko M, Sherman A, Eggan K, Henderson CE, Maragakis NJ.

Stem Cells Transl Med.

2013

Modifications of hippocampal circuits and early disruption of adult neurogenesis in the tg2576 mouse model of Alzheimer's disease.

Krezymon A, Richetin K, Halley H, Roybon L, Lassalle JM, Francès B, Verret L, Rampon C.

PLoS One

Human stem cell-derived spinal cord astrocytes with defined mature or reactive phenotypes.

Roybon L, Lamas NJ, Garcia-Diaz A, Yang EJ, Sattler R, Jackson-Lewis V, Kim YA, Kachel CA, Rothstein JD, Przedborski S, Wichterle H, Henderson CE.

Cell Reports

Accelerated high-yield generation of limb-innervating motor neurons from human stem cells.

Amoroso MW, Croft GF, Williams DJ, O'Keeffe S, Carrasco MA, Davis AR, Roybon L, Oakley DH, Maniatis T, Henderson CE, Wichterle H.

J Neurosci.

NGF rescues hippocampal cholinergic neuronal markers, restores neurogenesis, and improves the spatial working memory in a mouse model of Huntington's Disease.

Zhang H, Petit GH, Gaughwin PM, Hansen C, Ranganathan S, Zuo X, Smith R, Roybon L, Brundin P, Mobley WC, Li JY.

J Huntingtons Dis.

2012 and Before

The adult human brain harbors multipotent perivascular mesenchymal stem cells.

Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, Jansson K, Dannaeus K, Henriques-Oliveira C, Roybon L, Anisimov SV, Renström E, Svensson M, Haegerstrand A, Brundin P.

PLoS One

Neurogenesis in the R6/2 mouse model of Huntington's disease is impaired at the level of NeuroD1.

Fedele V, Roybon L, Nordström U, Li JY, Brundin P.

Neuroscience

Brain injury activates microglia that induce neural stem cell proliferation ex vivo and promote differentiation of neurosphere-derived cells into neurons and oligodendrocytes.

Deierborg T, Roybon L, Inacio AR, Pesic J, Brundin P.

Neuroscience

GABAergic differentiation induced by Mash1 is compromised by the bHLH proteins Neurogenin2, NeuroD1, and NeuroD2.

Roybon L, Mastracci TL, Ribeiro D, Sussel L, Brundin P, Li JY.

Cereb Cortex

Absence of striatal newborn neurons with mature phenotype following defined striatal and cortical excitotoxic brain injuries.

Deierborg T, Staflin K, Pesic J, Roybon L, Brundin P, Lundberg C.

Exp Neurol

Neurogenin2 directs granule neuroblast production and amplification while NeuroD1 specifies neuronal fate during hippocampal neurogenesis.

Roybon L, Hjalt T, Stott S, Guillemot F, Li JY, Brundin P.

PLoS One

Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis.

Roybon L, Deierborg T, Brundin P, Li JY.

Eur J Neurosci

How to build a brain: transcription factors generate interneuron diversity in the olfactory bulb (Commentary on Roybon et al.).

Fritschy JM.

Eur J Neurosci

Bone marrow multipotent mesenchymal stroma cells act as pericyte-like migratory vehicles in experimental gliomas.

Bexell D, Gunnarsson S, Tormin A, Darabi A, Gisselsson D, Roybon L, Scheding S, Bengzon J.

Mol Ther

Emerging restorative treatments for Parkinson's disease.

Deierborg T, Soulet D, Roybon L, Hall V, Brundin P.

Prog Neurobiol

Effects on differentiation of embryonic ventral midbrain progenitors by Lmx1a, Msx1, Ngn2, and Pitx3.

Roybon L, Hjalt T, Christophersen NS, Li JY, Brundin P.

The Journal of Neuroscience

Fibroblast growth factor-20 increases the yield of midbrain dopaminergic neurons derived from human embryonic stem cells.

Correia AS, Anisimov SV, Roybon L, Li JY, Brundin P.

Front Neuroanat.

Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: effect of in vitro differentiation on graft survival and teratoma formation.

Brederlau A, Correia AS, Anisimov SV, Elmi M, Paul G, Roybon L, Morizane A, Bergquist F, Riebe I, Nannmark U, Carta M, Hanse E, Takahashi J, Sasai Y, Funa K, Brundin P, Eriksson PS, Li JY.

Stem Cells

Failure of transdifferentiation of adult hematopoietic stem cells into neurons.

Roybon L, Ma Z, Asztely F, Fosum A, Jacobsen SE, Brundin P, Li JY.

Stem Cells

Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.

Roybon L, Brundin P, Li JY.

Exp Neurol

Stem cell therapy for Parkinson's disease: where do we stand?

Roybon L, Christophersen NS, Brundin P, Li JY.

Cell Tissue Res

Recognition of cell surface acceptors by two human alpha-2,6-sialyltransferases produced in CHO cells.

Donadio S, Dubois C, Fichant G, Roybon L, Guillemot JC, Breton C, Ronin C.